Using an aryl phenanthroimidazole moiety as a conjugated flexible intercalator to improve the hybridization efficiency of a triplex-forming oligonucleotide.

@article{Osman2008UsingAA,
  title={Using an aryl phenanthroimidazole moiety as a conjugated flexible intercalator to improve the hybridization efficiency of a triplex-forming oligonucleotide.},
  author={Amany Mostafa Ahmed Osman and Per Trolle J\orgensen and Niels Bomholt and Erik Bjerregaard Pedersen},
  journal={Bioorganic & medicinal chemistry},
  year={2008},
  volume={16 23},
  pages={9937-47}
}
When inserting 2-phenyl or 2-naphth-1-yl-phenanthroimidazole intercalators (X and Y, respectively) as bulges into triplex-forming oligonucleotides, both intercalators show extraordinary high thermal stability of the corresponding Hoogsteen-type triplexes and Hoogsteen-type parallel duplexes with high discrimination to Hoogsteen mismatches. Molecular modeling shows that the phenyl or the naphthyl ring stacks with the nucleobases in the TFO, while the phenanthroimidazol moiety stacks with the… CONTINUE READING