Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia rolled-leaf beetle (Coleoptera: Chrysomelidae)

@article{GarcaRobledo2013UsingAC,
  title={Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia rolled-leaf beetle (Coleoptera: Chrysomelidae)},
  author={Carlos Garc{\'i}a‐Robledo and Erin K. Kuprewicz and Charles L. Staines and W. John Kress and Terry L. Erwin},
  journal={Biological Journal of The Linnean Society},
  year={2013},
  volume={110},
  pages={189-198}
}
To fully understand the ecology and evolution of plant–herbivore interactions, information regarding the life history of both immature and adult insect stages is essential. However, most knowledge of plant–herbivore associations is derived from observations of adults. One reason for this bias is that species identification of immature stages is usually challenging. DNA barcodes can be used to identify immature stages to the species level. This technique compares short sequences of the… 

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References

SHOWING 1-10 OF 46 REFERENCES
Identification of net‐winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility
TLDR
It is demonstrated that high‐density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results.
Tropical Plant–Herbivore Networks: Reconstructing Species Interactions Using DNA Barcodes
TLDR
This study demonstrates that host plant identifications at the species-level using DNA barcodes are feasible, cost-effective, and reliable, and that reconstructing plant-herbivore networks with these methods will become the standard for a detailed understanding of these interactions.
Association of insect life stages using DNA sequences: the larvae of Philodytes umbrinus (Motschulsky) (Coleoptera: Dytiscidae)
TLDR
Three unknown larval specimens of an apparent species of Laccophilinae collected in Namibia were associated with the species Philodytes umbrinus (Motschulsky) using DNA sequence data, and the unknown larvae were assigned to P.Umbrinus.
Identifying Insects with Incomplete DNA Barcode Libraries, African Fruit Flies (Diptera: Tephritidae) as a Test Case
TLDR
A general working strategy to deal with incomplete reference libraries in the DNA barcoding identification of species by imposing a distance threshold profitably reduces identification errors is proposed.
DNA barcodes distinguish species of tropical Lepidoptera.
TLDR
It is shown that cytochrome c oxidase I DNA barcodes effectively discriminate among species in three Lepidoptera families from Area de Conservación Guanacaste in northwestern Costa Rica, suggesting DNA barcoding will significantly aid species identification and discovery in tropical settings.
Association of larvae and adults of Mexican species of Macrelmis (Coleoptera: Elmidae): A preliminary analysis using DNA sequences
TLDR
Adult/larval association in elmids using DNA sequence data seems to be promising in terms of speed and reliability.
DNA identification and morphological description of the first confirmed larvae of Hetaeriinae (Coleoptera: Histeridae)
TLDR
Using DNA sequences of nuclear ribosomal (18S) and mitochondrial (cytochrome oxidase I) genes (a modified DNA barcoding approach), larvae of hetaeriine Histeridae are positively identified for the first time.
DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success.
TLDR
An outlook on the prospects of obtaining complete barcode databases and the future use of DNA sequences in a modern integrative taxonomy is concluded.
Taxonomy: DNA barcodes: recent successes and future prospects
TLDR
Two recent studies convincingly demonstrate the efficacy of DNA barcoding to recover biologically significant groupings or species and suggest that accurate species identification is now possible by anyone with access to DNA sequencing even if they lack taxonomic expertise.
...
1
2
3
4
5
...