Using Xenopus skin to study cilia development and function.

  title={Using Xenopus skin to study cilia development and function.},
  author={Michael Edward Werner and Brian J Mitchell},
  journal={Methods in enzymology},
Cilia are prevalent biological structures that are important for cell signaling and for generating fluid flow (or motility). Cilia are found throughout biology from single-celled organisms to vertebrates, and many model systems have been employed for their analysis. Here, we describe the use of Xenopus larval skin as a system for the study of ciliogenesis and ciliary function. In particular, we describe basic molecular and embryological manipulations and imaging techniques that have proven… CONTINUE READING

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