Using Standard Optical Flow Cytometry for Synchronizing Proliferating Cells in the G1 Phase

Abstract

Cell cycle research greatly relies on synchronization of proliferating cells. However, effective synchronization of mammalian cells is commonly achieved by long exposure to one or more cell cycle blocking agents. These chemicals are, by definition, hazardous (some more than others), pose uneven cell cycle arrest, thus introducing unwanted variables. The… (More)
DOI: 10.1371/journal.pone.0083935

Topics

5 Figures and Tables

Slides referencing similar topics