Using Reduced Inoculum Densities of Mycobacterium tuberculosis in MGIT Pyrazinamide Susceptibility Testing to Prevent False-Resistant Results and Improve Accuracy: A Multicenter Evaluation

@inproceedings{Morlock2017UsingRI,
  title={Using Reduced Inoculum Densities of Mycobacterium tuberculosis in MGIT Pyrazinamide Susceptibility Testing to Prevent False-Resistant Results and Improve Accuracy: A Multicenter Evaluation},
  author={Glenn P Morlock and Frances C Tyrrell and Dorothy Baynham and Vincent E Escuyer and Nicole Green and Youngmi Kim and Patricia A Longley-Olson and Nicole Parrish and Courtney Pennington and Desmond Tan and Brett Austin and James E Posey},
  booktitle={Tuberculosis research and treatment},
  year={2017}
}
The primary platform used for pyrazinamide (PZA) susceptibility testing of Mycobacterium tuberculosis is the MGIT culture system (Becton Dickinson). Since false-resistant results have been associated with the use of this system, we conducted a multicenter evaluation to determine the effect of using a reduced cell density inoculum on the rate of false resistance. Two reduced inoculum densities were compared with that prescribed by the manufacturer (designated as "BD" method). The reduced… CONTINUE READING