Using Molecular Biology Tools to Identify Abscission Materials for Citrus

  title={Using Molecular Biology Tools to Identify Abscission Materials for Citrus},
  author={Jacqueline K. Burns},
  • J. Burns
  • Published 1 June 2002
  • Biology
  • Hortscience
Received for publication 29 Nov. 2001. Accepted for publication 30 Nov. 2001. This research was supported by the Florida Agricultural Experiment Station, and approved for publication as Journal Series No. R-08293. I gratefully acknowledge support by the Florida Dept. of Citrus and the Univ. of Florida for this work. Professor. A fundamental element of plant development is abscission of mature or ripe fruit, senescent tissues, or damaged organs. Abscission is a complex, organized, and regulated… 

Figures and Tables from this paper

Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus
This work identifies genes potentially involved in organ abscission and provides relevant data for future biotechnology approaches aimed at controlling such crucial process for citrus yield.
Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene
The underlying mechanisms of some known important biochemical events occurring at AZ-C are unraveled and should provide informative suggestions for future manipulation of the events to achieve a controllable abscission for mature citrus fruit.
Ethylene-induced differential gene expression during abscission of citrus leaves
expression data associated with the petiole indicated the occurrence of a double defensive strategy mediated by the activation of a biochemical programme including scavenging ROS, defence and PR genes, and a physical response mostly based on lignin biosynthesis and deposition.
Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves
This study provides novel molecular insight into ethylene-promoted leaf abscissions and identifies new putative target genes for characterization and manipulation of organ abscission in citrus.
A beta-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis).
The results suggest that this beta-galactosidase may play a role during abscission as well as early growth and development processes in flowers and fruitlets.
Potential Abscission Agents for Raisin, Table, and Wine Grapes
Methyl jasmonate (MeJA) and six other compounds known to stimulate abscission of other fruits were screened for their ability to reduce FDF of mature 'Thompson Seedless' grapes and MeJA was particularly effective.
Isolation and characterization of a cDNA encoding a lipid transfer protein expressed in 'Valencia' orange during abscission.
The results suggest that CsLTP has a role in the abscission process, possibly by assisting transport of cutin monomers to the fracture plane of the ab scission zone or through its anti-microbial activity by reducing the potential of microbial attack.
Organ Loss and Yield Impacts of ‘Valencia’ Sweet Orange in Response to Fruit Abscission Agents
The use of the abscission agent 5-chloro- 3-methyl-4-nitro-1H-pyrazole (CMNP) in combination with mechanical harvesting increases mature sweet orange fruit removal without causing phytotoxicity to leaves and young developing fruit through most of the harvesting season.
Expression of Ethylene Biosynthesis and Signaling Genes during Differential Abscission Responses of Sweet Orange Leaves and Mature Fruit
A BSTRACT . When applying abscission agents to tree fruit to facilitate harvest, it is desirable to loosen fruit and not leaves or other organs, but mechanisms controlling leaf and fruit drop are not
Genes for Hormone Biosynthesis and Regulation Are Highly Expressed in Citrus Flowers Infected with the Fungus Colletotrichum acutatum, Causal Agent of Postbloom Fruit Drop
Results indicate that infection by C. acutatum of citrus flowers triggered differential gene expressions, mainly associated with IAA, ethylene, and JA production and regulation, and increased hormone concentrations, consistent with the hypothesis of the involvement of phytohormones in postbloom fruit drop.


Cell Separation Processes in Plants—Models, Mechanisms and Manipulation
The use of transgenic plants and model systems such as Arabidopsis is assisting the understanding of the mechanisms that regulate abscission and dehiscence and the application of this information will advance understanding of cell separation processes in general.
Endo‐1,4‐β‐glucanase gene expression and cell wall hydrolase activities during abscission in Valencia orange
Genomic Southern analysis suggested that Valencia orange contained two groups of cellulase genes, which were expressed in ethylene-induced calyx, laminar and floral abscission zones, but were not expressed in non-induced abscissions zones or mature leaves treated with or without ethylene, young bark or young fruit of Valencia.
Citrus Fruit Abscission Induced by Methyl-jasmonate
ADDITIONAL INDEX WORDS. Citrus sinensis, 'Hamlin' orange, 'Valencia' orange, mechanical harvest, ethylene ABSTRACT. Methyl jasmonate (MJ) was tested as a potential abscission chemical to enhance
The jasmonate pathway is involved differentially in the regulation of different defence responses in tobacco cells
The obtained results indicate that synthesis of jasmonate via the LOX pathway seems to be only part of a complex regulatory mechanism for the onset of many, but not all, defence reactions.
Jasmonates promote abscission in bean petiole expiants: Its relationship to the metabolism of cell wall polysaccharides and cellulase activity
It is reported that JA and JA-Me promote abscission in bean petiole expiants and that the changes in the metabolism of cell wall polysaccharides in thePetiole and the pulvinus adjacent to the abscissions zone are involved in the promotive effects of these compounds.
Ethylene: a gaseous signal molecule in plants.
Biotechnological modifications of ethylene synthesis and of sensitivity to ethylene are promising methods to prevent spoilage of agricultural products such as fruits, whose ripening is induced by ethylene.
Stimulation of ethylene production in bean leaf discs by the pseudomonad phytotoxin coronatine.
Results of experiments using l-[U-(14)C]methionine, indicated that the stimulation involved de novo production of ethylene via the methionine pathway, which may have a specific role in the coronatine toxic syndrome.
A role for jasmonates in climacteric fruit ripening
Jasmonates may play a role together with ethylene in regulating the early steps of climacteric fruit ripening, indicating jasmonate act at least in part via ethylene action.
iaglu, a gene from Zea mays involved in conjugation of growth hormone indole-3-acetic acid.
Protein synthesized by Escherichia coli that contained cloned 1-O-beta-D-indol-3-ylacetyl-glucose complementary DNA (cDNA) was catalytically active and the predicted amino acid sequence of the cDNA was confirmed by amino-terminal sequencing of the purified enzyme.
Chlorosis-inducing Phytotoxins Produced by Pseudomonas syringae
  • C. Bender
  • Biology
    European Journal of Plant Pathology
  • 2004
The utilization of toxin resistance genes in the development of transgenic plants with phytotoxin tolerance is reviewed and the mode of action, genetics, biosynthesis and regulation of coronatine, tabtoxin, and phaseolotoxin is reviewed.