Using Klenow-mediated extension to measure poly(A)-tail length and position in the transcriptome.

Abstract

The poly(A)-tail that terminates most mRNA and many noncoding RNA is a convenient "hook" to isolate mRNA. However the length of this tail and its position within the primary RNA transcript can also hold diagnostic value for RNA metabolism. In general, mRNA with a long poly(A)-tail is well translated, whereas a short poly(A)-tail can indicate translational… (More)
DOI: 10.1007/978-1-62703-971-0_3

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Cite this paper

@article{Lee2014UsingKE, title={Using Klenow-mediated extension to measure poly(A)-tail length and position in the transcriptome.}, author={Man Chun Lee and Amrei J{\"a}nicke and Traude H Beilharz}, journal={Methods in molecular biology}, year={2014}, volume={1125}, pages={25-42} }