Using Co-Cultures Expressing Fluorescence Resonance Energy Transfer Based Protein Biosensors to Simultaneously Image Caspase-3 and Ca2+ Signaling

@article{Chiang2005UsingCE,
  title={Using Co-Cultures Expressing Fluorescence Resonance Energy Transfer Based Protein Biosensors to Simultaneously Image Caspase-3 and Ca2+ Signaling},
  author={Jason Chiang and Kevin Truong},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={1219-1227}
}
Fluorescence resonance energy transfer (FRET)-based protein biosensors allow the spatial and temporal imaging of signaling events in living cells. However, the simultaneous correlation of multiple events of a signaling pathway is hindered by the spectral cross-talk between fluorescent proteins. Here, we show, for signaling pathways that progress… CONTINUE READING