Using CTLL-2 and CTLL-2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test.

@article{Meintires2003UsingCA,
  title={Using CTLL-2 and CTLL-2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test.},
  author={Sophie Meinti{\`e}res and Armelle Biola and Marc Pallardy and Daniel Marzin},
  journal={Environmental and molecular mutagenesis},
  year={2003},
  volume={41 1},
  pages={14-27}
}
In vitro assays for chromosome aberrations (i.e., in vitro micronucleus and in vitro metaphase analysis tests) frequently produce false-positive or exaggerated-positive results. Our previous work suggested that apoptosis interferes with these tests, producing misleading results. These previous studies were conducted by performing the in vitro micronucleus test in CTLL-2 cells and a CTLL-2 cell derivative stably transfected with the apoptosis inhibitor gene bcl2. In the present study, these… CONTINUE READING