Uses of isotope effects in the study of enzymes.

  title={Uses of isotope effects in the study of enzymes.},
  author={Dexter B. Northrop},
  volume={24 2},
There have been few recent additions to the technical methods employed in the study of isotope effects, notable exceptions being the use of high pressure as an experimental variable and the measurement of heavy-atom isotope effects on maximal velocities using continuous-flow techniques. Most of the innovations are in the realm of new experimental designs that allow the asking of new questions. These designs include the use of isotope effects to: determine kinetic mechanisms, distinguish between… CONTINUE READING