Usefulness of supernatant of pancreatic juice for genetic analysis of K-ras in diagnosis of pancreatic carcinoma.

@article{Ha2001UsefulnessOS,
  title={Usefulness of supernatant of pancreatic juice for genetic analysis of K-ras in diagnosis of pancreatic carcinoma.},
  author={Andrew Ha and Hiroyuki Watanabe and Yasushi Yamaguchi and Koushiro Ohtsubo and Y Z Wang and Yoshiharu Motoo and Takashi Okai and T Wakabayahi and Norio Sawabu},
  journal={Pancreas},
  year={2001},
  volume={23 4},
  pages={356-63}
}
AIMS To ascertain whether analysis of K-ras mutations at codon 12 (KRM) in the supernatant of pure pancreatic juice (PPJ) is more useful for the diagnosis of pancreatic carcinoma (PCa) than that in sediment, the authors analyzed KRM in DNA extract from not only the sediment but also the supernatant of PPJ and compared the results. METHODOLOGY PPJ was collected endoscopically from 19 patients with PCa and 25 patients with chronic pancreatitis (CP). DNA was extracted from the supernatant and… CONTINUE READING

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