Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro-derived bovine blastocysts.

@article{Ohboshi1997UsefulnessOP,
  title={Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro-derived bovine blastocysts.},
  author={Shigeki Ohboshi and Noboru Fujihara and Takeshi Yoshida and Hiroshi Tomogane},
  journal={Animal reproduction science},
  year={1997},
  volume={48 1},
  pages={
          27-36
        }
}
A series of five experiments measured the high survival of bovine blastocysts produced in vitro after cryopreservation by vitrification. The vitrification solution (designated VS) contained 40% (v/v) ethylene glycol, 6% (w/v) polyethylene glycol and 0.5 M sucrose in phosphate-buffered saline. Embryos developed in vitro at Days 7 and 8 (Day 0 = insemination day) were exposed in one step to VS for 1 min or two steps with 10% ethylene glycol for 5 min and then VS for 1 min. In both cases, the… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-9 OF 9 CITATIONS

Using a Novel Supramolecular Gel Cryopreservation System in Microchannel to Minimize the Cell Injury.

  • Langmuir : the ACS journal of surfaces and colloids
  • 2018
VIEW 2 EXCERPTS
CITES BACKGROUND

In-straw cryoprotectant dilution for bovine embryos vitrified using Cryotop.

  • The Journal of reproduction and development
  • 2011
VIEW 1 EXCERPT
CITES RESULTS

Factors affecting the outcome of human blastocyst vitrification

  • Reproductive biology and endocrinology : RB&E
  • 2009
VIEW 1 EXCERPT
CITES METHODS

Cryopreservation of Mammalian Embryos

Vitrification Shigeki
  • 2007