Use of whole sheep red blood cells in ELISA to assess immunosuppression in vivo.

  title={Use of whole sheep red blood cells in ELISA to assess immunosuppression in vivo.},
  author={Ai Koganei and T Tsuchiya and Keiji Samura and Masaru Nishikibe},
  journal={Journal of immunotoxicology},
  volume={4 1},
An enzyme-linked immunosorbent assay (ELISA) using whole sheep red blood cells (SRBC) has been reported as one of the methods for detecting a T-lymphocyte-dependent antibody response. However, it has not been widely used because of SRBC problems such as the weak attachment to ELISA plates, specificity and short-term stability. The objectives of this study were to address these issues and to validate the SRBC-specific antibody response assay. Male Sprague-Dawley rats were bled after 6 days of… CONTINUE READING


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