Use of weak acids to determine the bulk diffusion limitation of H+ ion conductance through the gramicidin channel.

@article{Decker1988UseOW,
  title={Use of weak acids to determine the bulk diffusion limitation of H+ ion conductance through the gramicidin channel.},
  author={E. Radford Decker and David G. Levitt},
  journal={Biophysical journal},
  year={1988},
  volume={53 1},
  pages={25-32}
}
The addition of 2 M formic acid at pH 3.75 increased the single channel H+ ion conductance of gramicidin channels 12-fold at 200 mV. Other weak acids (acetic, lactic, oxalic) produce a similar, but smaller increase. Formic acid (and other weak acids) also blocks the K+ conductance at pH 3.75, but not at pH 6.0 when the anion form predominates. This increased H+ conductance and K+ block can be explained by formic acid (HF) binding to the mouth of the gramicidin channel (Km = 1 M) and providing a… CONTINUE READING

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