Use of voltage-sensitive dyes and optical recordings in the central nervous system

@article{Ebner1995UseOV,
  title={Use of voltage-sensitive dyes and optical recordings in the central nervous system},
  author={T. Ebner and G. Chen},
  journal={Progress in Neurobiology},
  year={1995},
  volume={46},
  pages={463-506}
}
Understanding the spatio-temporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Electrophysiological and other mapping techniques have provided important insights into the function of neural circuits and neural populations in many systems. However, there remain limitations with these approaches. Therefore, complementary techniques which permit the monitoring of the spatio-temporal… Expand

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References

SHOWING 1-10 OF 290 REFERENCES
Imaging of cerebellar surface activation In vivo using voltage sensitive dyes
TLDR
The use of voltage sensitive dyes and image processing techniques to monitor in vivo the activation of parallel fibers and associated neuronal events produced by stimulation of the cerebellar cortex in the rat are described. Expand
Real-time optical imaging of naturally evoked electrical activity in intact frog brain
TLDR
It is demonstrated that optical measurements can be used for real-time imaging of spatio-temporal patterns of neuronal responses and for identification of functional units evoked by natural visual stimuli. Expand
Voltage-sensitive dyes and functional activity in the olfactory pathway.
TLDR
This review summarizes results obtained with voltage-sensitive dye, a method having good spatial and temporal resolution for observ­ing distributed neuronal activity, as it has been applied to the olfactory pathway. Expand
Real-time imaging of evoked activity in local circuits of the salamander olfactory bulb
TLDR
A video method for imaging voltage-sensitive dye fluorescence in vivo is used to map neuronal activity in local circuits of the salamander olfactory bulb and provides high resolution, sequential video images of the spatial and temporal progression of transmembrane events in neuronal circuits after afferent stimulation. Expand
Optical measurements of potential changes in axons and processes of neurons of a barnacle ganglion
  • W. N. Ross, V. Krauthamer
  • Biology, Medicine
  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1984
TLDR
This comparison demonstrated that signal-averaged records with large signal-to-noise ratios were obtained from those parts of the visual field which contained elements of the stimulated cell and hence could be attributed to specific locations on the cell. Expand
An apparatus for recording synaptic potentials from neuronal cultures using voltage-sensitive fluorescent dyes
  • C. Chien, J. Pine
  • Medicine, Materials Science
  • Journal of Neuroscience Methods
  • 1991
TLDR
A system for recording from microcultures of 1-15 neurons from the rat superior cervical ganglion, using voltage-sensitive fluorescent dyes of the styryl class, which should be useful for studying long-term synaptic plasticity in cultures of vertebrate neurons. Expand
Optical recording of voltage changes in nerve terminals and in fine neuronal processes.
  • B. Salzberg
  • Materials Science, Medicine
  • Annual review of physiology
  • 1989
TLDR
It is shown that changes in membrane potential from regions of an isolated cell with linear dimensions of approximately one micron can be measured and optical sectioning, with near elimination of scattering by means of confocal imaging, may soon permit optical recording from compar­ ably small regions of cells in intact ganglia or brains. Expand
Real-time optical mapping of neuronal activity: from single growth cones to the intact mammalian brain.
  • A. Grinvald
  • Biology, Medicine
  • Annual review of neuroscience
  • 1985
Electrophysiology is at present the most commonly used approach with both the spatial and the temporal resolution required to investigate the real time function of individual neurons or of neuronalExpand
Simultaneous optical measurements of electrical activity from multiple sites on processes of cultured neurons.
TLDR
Optical methods using changes in fluorescence and absorption of voltage-sensitive dyes were developed to record electrical activity from processes of nerve cells grown in monolayer culture and a merocyanine dye was discovered but was more sensitive than others previously tested on cultured neurons. Expand
Voltage-sensitive dye recording of action potentials and synaptic potentials from sympathetic microcultures.
TLDR
The results show that voltage-sensitive dyes can be used to record from microcultures of vertebrate neurons with high sensitivity, and it should be possible to use dye recording to routinely measure subthreshold synaptic strengths. Expand
...
1
2
3
4
5
...