Use of truncated pyramid representation methodology in three-dimensional reconstruction: an example.

@article{Papadimitriou2004UseOT,
  title={Use of truncated pyramid representation methodology in three-dimensional reconstruction: an example.},
  author={Christos Papadimitriou and Christos Yapijakis and Panagiota Davaki},
  journal={Journal of microscopy},
  year={2004},
  volume={214 Pt 1},
  pages={70-5}
}
This paper describes a new methodology for three-dimensional (3D) representation of biological structures contained in a series of sections, using an illustrative example. Spatial reconstruction of a specific area of an astrocytoma biopsy was carried out with alignment of the serial sections at an accuracy of 0.01% (or 1 micro m cm(-1)), using the truncated pyramid representation (TPR) methodology. TPR includes: (a) serial tissue sectioning in a ribbon form; (b) alignment of the serial sections… CONTINUE READING

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