Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis.

@article{Rabilloud1998UseOT,
  title={Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis.},
  author={Thierry Rabilloud},
  journal={Electrophoresis},
  year={1998},
  volume={19 5},
  pages={
          758-60
        }
}
The separation of membrane proteins by high-resolution two-dimensional electrophoresis was carried out. At high loads, these proteins are prone to precipitation, resulting in poor resolution. It is shown here that the use of thiourea, previously described for focusing in immobilized pH gradients, can be extended to conventional isoelectric focusing. As thiourea inhibits acrylamide polymerization, a modified photopolymerization system must be used. These modifications result in higher solubility… CONTINUE READING
BETA

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 122 CITATIONS

FILTER CITATIONS BY YEAR

1999
2018

CITATION STATISTICS

  • 15 Highly Influenced Citations

  • Averaged 5 Citations per year over the last 3 years

Similar Papers

Loading similar papers…