Use of the refractive index to measure the adequacy of glycerol removal from previously frozen erythrocytes.

Abstract

A common method for measuring deglycerolization of previously frozen erythrocytes is to determine the osmolality of the supernatant fluid after completion of the wash cycle. We attempted to compare this type of examination with the simpler, faster, and cheaper determination of refractive index (RI) using the Goldberg refractometer. One hundred twenty units… (More)

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