Use of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations: possible implications of confounding factors.

@article{Atienzar2002UseOT,
  title={Use of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations: possible implications of confounding factors.},
  author={Franck A Atienzar and Andrew J Evenden and Awadhesh N Jha and Michael Depledge},
  journal={Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals},
  year={2002},
  volume={7 1},
  pages={94-101}
}
The aim of this study was to evaluate the potential of the random amplified polymorphic DNA (RAPD) assay to qualitatively detect the kinetics of benzo[a]pyrene (B[a]P)-induced DNA effects in the water flea Daphnia magna exposed to 25 and 50 micrograms l-1 B[a]P for 7 and 6 days, respectively. Mortality was recorded on a daily basis in both experiments, and RAPD analysis was performed on samples collected every day following isolation of genomic DNA. The main changes occurring in RAPD profiles… CONTINUE READING

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