Use of the polymerase chain reaction to identify coding sequences for chitin synthase isozymes in Phialophora verrucosa

Abstract

Based on conserved amino-acid regions predicted for the chitin synthases (Chs) of Saccharomyces cerevisiae, two different primer sets were synthesized and used in polymerase chain reactions (PCRs) to amplify 614-bp and 366-bp sequences from genomic DNA of the zoopathogenic fungus Phialophora verrucosa. DNA-sequencing and Southern-blotting analyses of the… (More)
DOI: 10.1007/BF00314441

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