Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes

@article{SerraMoreno2006UseOT,
  title={Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes},
  author={Ruth Serra-Moreno and Sandra Acosta and Jean Pierre Hernalsteens and Juan Jofre and Maite Muniesa},
  journal={BMC Molecular Biology},
  year={2006},
  volume={7},
  pages={31 - 31}
}
BackgroundThe Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in E. coli K-12 through homologous recombination using linear PCR products. The aim of this study was to induce mutations in the genome of some temperate Shiga toxin encoding bacteriophages. When phage genes are in the prophage state, they behave like chromosomal genes. This enables marker genes, such as antibiotic resistance genes, to be incorporated into the stx gene. Once the phages… CONTINUE READING

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Closed Genome Sequence of Escherichia coli K-12 Group Strain C600

  • Microbiology resource announcements
  • 2019
VIEW 1 EXCERPT
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 49 REFERENCES

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2000
VIEW 10 EXCERPTS
HIGHLY INFLUENTIAL

Prophages and bacterial genomics: what have we learned so far?

  • Molecular microbiology
  • 2003
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL