Use of the fluorescent probe LAURDAN to label and measure inner membrane fluidity of endospores of Clostridium spp.

@article{Hofstetter2012UseOT,
  title={Use of the fluorescent probe LAURDAN to label and measure inner membrane fluidity of endospores of Clostridium spp.},
  author={Simmon Hofstetter and Christian Denter and Roland Winter and lynn M. McMulleN and Michael G G{\"a}nzle},
  journal={Journal of microbiological methods},
  year={2012},
  volume={91 1},
  pages={93-100}
}
A method for measuring the fluidity of inner membranes of populations of endospores of Clostridium spp. with a fluorescent dye was developed. Cells of Clostridium beijerinckii ATCC 8260 and Clostridium sporogenes ATCC 7955 were allowed to sporulate in the presence of 6-dodecanoyl-2-dimethylaminonaphthalene (LAURDAN) on a soil-based media. Labeling of endospores with LAURDAN did not affect endospore viability. Removal of the outer membranes of endospores was done using a chemical treatment and… CONTINUE READING
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