Use of the comet assay to identify cells sensitive to tirapazamine in multicell spheroids and tumors in mice.

@article{Olive1996UseOT,
  title={Use of the comet assay to identify cells sensitive to tirapazamine in multicell spheroids and tumors in mice.},
  author={Peggy L. Olive and C M Vikse and Judit P. Ban{\'a}th},
  journal={Cancer research},
  year={1996},
  volume={56 19},
  pages={4460-3}
}
Tirapazamine, a bioreductive drug preferentially toxic to hypoxic cells, produces significant numbers of DNA single-strand breaks that can be detected using the alkaline comet assay. Our goal was to determine whether single-strand breaks measured using this assay could act as a surrogate end point for cell killing in multicell spheroids and solid tumors in mice. In spheroids composed of Chinese hamster V79 cells, WiDr human colon carcinoma cells, or SiHa human cervical carcinoma cells… CONTINUE READING
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