Use of the Tn903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe

@article{LangHinrichs1990UseOT,
  title={Use of the Tn903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe},
  author={Christine Dr. Lang-Hinrichs and Claudia D{\"o}ssereck and Isabelle Fath and U. Stahl},
  journal={Current Genetics},
  year={1990},
  volume={18},
  pages={511-516}
}
SummaryThe bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro aminoglycoside… CONTINUE READING

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SHOWING 1-2 OF 2 REFERENCES

Gene 50:225-237 C o m m u n i c a t e d by K

J Zhu, R Contreras, W Fiers
  • 1983