Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines.

@article{Grenier2002UseOT,
  title={Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines.},
  author={Simon Grenier and Silvia Maria Gomes and Bernard Pintureau and François Lassabli{\'e}re and Patrice Bolland},
  journal={Journal of invertebrate pathology},
  year={2002},
  volume={80 1},
  pages={
          13-21
        }
}
Endosymbionts of the genus Wolbachia were efficiently cured from Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no Wolbachia organisms were detected by PCR for up to 14 generations after curing. Four cured strains of Trichogramma pretiosum showed a significantly lower total fecundity compared to their Wolbachia-infected counterpart. However, the… CONTINUE READING

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