Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.

@article{LocatelliHoops2014UseOT,
  title={Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.},
  author={Silvia C Locatelli-Hoops and Alexei A. Yeliseev},
  journal={Methods in molecular biology},
  year={2014},
  volume={1177},
  pages={107-20}
}
Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion… CONTINUE READING