Use of stroma-supported cultures of leukemic cells to assess antileukemic drugs. II. Potent cytotoxicity of 2-chloro-deoxyadenosine in acute lymphoblastic leukemia.

@article{Kumagai1994UseOS,
  title={Use of stroma-supported cultures of leukemic cells to assess antileukemic drugs. II. Potent cytotoxicity of 2-chloro-deoxyadenosine in acute lymphoblastic leukemia.},
  author={Masa-aki Kumagai and Atsushi Manabe and Elaine Coustan-Smith and Raymond L. Blakley and William Beck and Victor M. Santana and Frederick G. Behm and Susana C. Raimondi and Dario Campana},
  journal={Leukemia},
  year={1994},
  volume={8 7},
  pages={
          1116-23
        }
}
We used a recently established stroma-supported tissue culture technique that allows long-term culture of acute lymphoblastic leukemia (ALL) cells to study 2-chloro-2'-deoxyadenosine (2CdA) cytotoxicity to leukemic lymphoblasts. In the 20 cases of ALL studied, the number of cells recovered after 7 days of culture on allogeneic stromal layers were 58-192% (median, 95.5%) of those originally seeded. In parallel cultures with 2CdA (100 nM), 74- > 99% (median, 97.5%) of leukemic lymphoblasts were… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations