Use of specific lysine modifications to locate the reaction site of cytochrome c with cytochrome oxidase.

@article{Smith1977UseOS,
  title={Use of specific lysine modifications to locate the reaction site of cytochrome c with cytochrome oxidase.},
  author={H. J. T. Smith and Nancy S. Staudenmayer and F S Millett},
  journal={Biochemistry},
  year={1977},
  volume={16 23},
  pages={4971-4}
}
The reaction of cytochrome c with trifluoromethylphenyl isocyanate was carried out under conditions which led to the modification of a small number of the 19 lysines. Extensive ion-exchange chromatography was used to separate and purify six different derivatives, each modified at a single lysine residue, lysines 8, 13, 27, 72, 79, and 100, respectively. The only modifications which affected the activity of cytochrome c with cytochrome oxidase (EC 1.9.3.1) were those of lysines immediately… CONTINUE READING