Use of siderophores to type pseudomonads: the three Pseudomonas aeruginosa pyoverdine systems.
@article{Meyer1997UseOS,
title={Use of siderophores to type pseudomonads: the three Pseudomonas aeruginosa pyoverdine systems.},
author={J. M. Meyer and Alain Stintzi and Daniel De Vos and Pierre Cornelis and Robert Tappe and Kambiz Taraz and Herbert Budzikiewicz},
journal={Microbiology},
year={1997},
volume={143 ( Pt 1)},
pages={
35-43
}
}Eighty-eight Pseudomonas aeruginosa isolates, most of them from the Collection of Bacterial Strains of the Institut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-facilitated iron uptake. The same grouping of the strains was reached by each of these methods…
227 Citations
Distribution and evolution of ferripyoverdine receptors in Pseudomonas aeruginosa.
- BiologyEnvironmental microbiology
- 2009
Molecular analyses of fpvA and fpVB genes highlighted a complex evolutionary history, probably linked to the central role of iron acquisition in the ecology and virulence of P. aeruginosa.
Siderotyping of fluorescent pseudomonads: characterization of pyoverdines of Pseudomonas fluorescens and Pseudomonas putida strains from Antarctica.
- BiologyMicrobiology
- 1998
The conclusion that the Antarctic Pseudomonas strains have no special features at the level of their pyoverdines and py overdine-mediated iron metabolism compared to worldwide strains demonstrates that siderotyping provides a rapid means of screening for novel pyOverdines.
Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa.
- BiologyMicrobiology
- 2003
Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA, which is encoded by a mini-TnphoA3 transposon.
Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa
- BiologyJournal of bacteriology
- 2005
The pyoverdine region shows evidence of horizontal transfer and it is proposed that some alleles in the region were introduced from other soil bacteria and have been subsequently maintained by diversifying selection.
Rapid Identification of Pseudomonas spp. via Raman Spectroscopy Using Pyoverdine as Capture Probe.
- Biology, ChemistryAnalytical chemistry
- 2016
It is proved that pyoverdine based isolation of bacteria is fully Raman compatible and further investigated the capability of this approach by isolating and identifying P. aeruginosa and P. fluorescens from tap water samples, which are both opportunistic pathogens and can pose a threat for immunocompromised patients.
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
- BiologyMicrobiology
- 2003
It is likely that most if not all of the genes required for pyoverdine synthesis in P. aeruginosa PAO1 have now been identified and this will form the basis for a biochemical description of the pathway of pyoverDine synthesis.
Pyoverdine biosynthesis and secretion in Pseudomonas aeruginosa: implications for metal homeostasis.
- BiologyEnvironmental microbiology
- 2013
The cellular localization of pyoverdine precursors was recently shown to be consistent with their biosynthetic enzymes, and a recently identified ATP-dependent efflux pump, PvdRT-OpmQ, is considered, which contributes to the understanding of the role of py overdine in iron and metal homeostasis in fluorescent Pseudomonads.
Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Pseudomonas putida.
- BiologyInternational journal of systematic and evolutionary microbiology
- 2007
The results strongly support the view that pyoverdine-based siderotyping could be used as a powerful tool in Pseudomonas taxonomy.
Application of Siderotyping for Characterization of Pseudomonas tolaasii and “Pseudomonas reactans” Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms
- Biology, MedicineApplied and Environmental Microbiology
- 2000
The easy and rapid methods of siderotyping proved to be reliable by supporting and strengthening previous taxonomical data and found an identity for some “P. reactans” isolates, with reference strains belonging to theP.
References
SHOWING 1-10 OF 47 REFERENCES
Evidence for different pyoverdine-mediated iron uptake systems among Pseudomonas aeruginosa strains
- BiologyInfection and immunity
- 1989
Two new types of pyoverdines produced by strains belonging to the Pseudomonas aeruginosa species were characterized and suggested the existence of three specificity groups.
Pyoverdin is essential for virulence of Pseudomonas aeruginosa
- Biology, MedicineInfection and immunity
- 1996
It is suggested that pyoverdin competes directly with transferrin for iron and that it is an essential element for in vivo iron gathering and virulence expression in P. aeruginosa.
Structure elucidation of a Pyoverdin Produced by Pseudomonas aeruginosa ATCC 27 853
- Chemistry
- 1993
The primary structure of a pyoverdin (Pa TII) isolated from an iron deficient culture medium of Pseudomonas aeruginosa ATCC 27 853 has been elucidated by spectroscopic methods and degradation…
Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin
- BiologyInfection and immunity
- 1985
A combination of the siderophores produced by Pseudomonas aeruginosa, pyochelin and pyoverdin, dramatically stimulates the growth of this bacterium in medium containing human transferrin. The amount…
Acquisition of iron from host sources by mesophilic Aeromonas species.
- BiologyJournal of general microbiology
- 1991
Most isolates (regardless of siderophore produced) used haem as a sole source of iron for growth; all of 33 isolates grew with either haematin or haemoglobin and 30 of these usedHaemoglobin when complexed to human haptoglobin.
Pyoverdin‐facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdin receptor
- BiologyMolecular microbiology
- 1990
A purified polyclonal antiserum directed against the isolated main 80kD IROMP from Pseudomonas aeruginosa PAO1 detected only the 80 kD polypeptide of outer‐membrane proteins from PAO 1 cells grown in iron deficiency in Western blots, and it was shown to inhibit the uptake of 59Fe pyoverdin byPAO1 cells as well as its binding to purified outer membranes.
Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa.
- Biology, MedicineInfection and immunity
- 1986
Bacteria appeared to be aiding the siderophores in iron mobilization from transferrin, as no pyochelin-mediated iron mobilization could be detected at pH 7.4, and there was transport.
Detection of the outer membrane lipoprotein I and its gene in fluorescent and non-fluorescent pseudomonads: implications for taxonomy and diagnosis.
- BiologyJournal of general microbiology
- 1993
The open reading frame of the OprI lipoprotein gene from Pseudomonas aeruginosa was amplified by polymerase chain reaction (PCR) starting from purified DNA or colony lysates and demonstrated a sequence variation in the oprI gene.
Two simple media for the demonstration of pyocyanin and fluorescin.
- Biology
- 1954
Abstract Two simple media for the enhancement of pigment production by certain organisms of the Pseudomonas genus are described. The results of comparative studies employing these media, certain…
Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae
- BiologyInfection and immunity
- 1988
The ability of V. cholerae to utilize mammalian iron compounds was determined, and it was found that both hemin and hemoglobin could serve as sole sources of iron.





