Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species

@article{Smulders1997UseOS,
  title={Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species},
  author={Marinus J M Smulders and G. M. M. Bredemeijer and W. Rus-Kortekaas and Paul Arens and Ben Vosman},
  journal={Theoretical and Applied Genetics},
  year={1997},
  volume={94},
  pages={264-272}
}
Abstract A search of nearly 2000 sequences from Solanaceae species in the EMBL and Genbank databases yielded 220 microsatellites. Among these were 80 microsatellites from 675 Lycopersicon entries. Dinucleotide repeats, as well as (CAA)n and (TAA)n repeats, were over-represented in non-coding DNA. The other trinucleotide repeats were predominantly found in exonic DNA. PCR analysis of 44 of the microsatellite-containing Lycopersicon loci identified 36 primer pairs that yielded well-scorable… CONTINUE READING

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