Use of sequential chemical extractions to purify nuclear membrane proteins for proteomics identification.

@article{Korfali2009UseOS,
  title={Use of sequential chemical extractions to purify nuclear membrane proteins for proteomics identification.},
  author={Nadia Korfali and Elizabeth A. L. Fairley and Selene K Swanson and Laurence Florens and Eric C Schirmer},
  journal={Methods in molecular biology},
  year={2009},
  volume={528},
  pages={201-25}
}
The nuclear envelope (NE) is a double membrane system that is both a part of the endoplasmic reticulum and part of the nucleus. As its constituent proteins tend to be highly complexed with nuclear and cytoplasmic components, it is notoriously difficult to purify. Two methods can reduce this difficulty for the identification of nuclear membrane proteins: comparison to contaminating membranes and chemical extractions to enrich for certain groups of proteins. The purification of nuclear envelopes… CONTINUE READING

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