Use of reversed-phase high-performance liquid chromatography on polystyrene-divinylbenzene columns for the rapid separation and purification of acid-soluble nuclear proteins.

@article{Zhelev1997UseOR,
  title={Use of reversed-phase high-performance liquid chromatography on polystyrene-divinylbenzene columns for the rapid separation and purification of acid-soluble nuclear proteins.},
  author={Nikolai Z Zhelev and Michael J. Barratt and Louis C. Mahadevan},
  journal={Journal of chromatography. A},
  year={1997},
  volume={763 1-2},
  pages={65-70}
}
The suitability of polystyrene-divinylbenzene reversed-phase HPLC columns for rapid separation and purification of acid-soluble nuclear proteins was evaluated. We used a polystyrene-divinylbenzene reversed-phase HPLC column (PLRP-S) for purification of nuclear proteins extracted with 0.3 M HCl or 5% HClO4. We are able to obtain electrophoretically pure… CONTINUE READING