Use of repetitive sequences to identify DNA polymorphisms linked to regA, a developmentally important locus in Volvox.

@article{Harper1987UseOR,
  title={Use of repetitive sequences to identify DNA polymorphisms linked to regA, a developmentally important locus in Volvox.},
  author={Jeffrey F. Harper and K S Huson and David B. Kirk},
  journal={Genes & development},
  year={1987},
  volume={1 6},
  pages={573-84}
}
The regA locus plays a centrally important role in Volvox development by preventing somatic cells from redifferentiating as germ cells; until now, approaches to cloning regA, as a preliminary to molecular analysis of its function, have been lacking. Here a novel approach is described that uses repetitive-sequence probes to rapidly identify restriction fragment length polymorphisms (RFLPs) linked to regA. Genomic DNA was cut with restriction enzymes having 4-base recognition sequences and then… CONTINUE READING