Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies.

@article{Rajeevan2001UseOR,
  title={Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies.},
  author={Mangalathu S. Rajeevan and Daya G Ranamukhaarachchi and Suzanne D. Vernon and Elizabeth R. Unger},
  journal={Methods},
  year={2001},
  volume={25 4},
  pages={443-51}
}
Real-time reverse transcription polymerase chain reaction (RT-PCR) methods that monitor product accumulation were adapted for the validation of differentially expressed genes. We describe a real-time quantitative PCR assay that uses SYBR Green I dye-based detection and product melting curve analysis to validate differentially expressed genes identified by gene expression profiling technologies. Since SYBR Green I dye is a nonspecific intercalating dye, the reaction is made specific by using… CONTINUE READING

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