Use of real time pulsed field gel electrophoresis to guide interventions during a nursery outbreak of Serratia marcescens infection.

@article{Hoyen1999UseOR,
  title={Use of real time pulsed field gel electrophoresis to guide interventions during a nursery outbreak of Serratia marcescens infection.},
  author={Claudia M Hoyen and Lawrence Rice and Silvia Conte and Michael R. Jacobs and Michele C Walsh-Sukys and Philip Toltzis},
  journal={The Pediatric infectious disease journal},
  year={1999},
  volume={18 4},
  pages={357-60}
}
BACKGROUND Pulsed field gel electrophoresis (PFGE) is a commercially available technique that can establish clonal relationships among many common hospital-derived organisms with a high degree of accuracy and can yield results in a sufficiently short time to guide interventions during an outbreak investigation. METHODS The CHEF Genomic Bacterial DNA Plug Kit (Bio-Rad) was applied to an unfolding nursery outbreak of Serratia marcescens infections according to the manufacturer's guidelines… CONTINUE READING