Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis.

  title={Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis.},
  author={Masahiro Kami and Toshiya Fukui and Seishi Ogawa and Yukumasa Kazuyama and Utako Machida and Yuji Tanaka and Yoshinobu Kanda and Takeshi Kashima and Yuji Yamazaki and Tamae Hamaki and Stefano Mori and Hisanori Akiyama and Yoshitomo Mutou and Hisashi Sakamaki and Kazuoki Osumi and Satoshi Kensuke Kimura and Hisamaru Hirai},
  journal={Clinical infectious diseases : an official publication of the Infectious Diseases Society of America},
  volume={33 9},
We developed a new quantitative system for diagnosis of invasive pulmonary aspergillosis (IPA) using real-time automated polymerase chain reaction (PCR). Intra-assay and interassay precision rates for in vitro examination were 2.53% and 2.20%, respectively, and the linearity of this assay was obtained when there were >20 copies/well. We examined 323 samples taken from 122 patients with hematological malignancies, including 33 patients with IPA and 89 control patients. Blood samples were… CONTINUE READING


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Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood

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