Use of rapidly mineralising osteoblasts and short periods of mechanical loading to accelerate matrix maturation in 3D scaffolds.

@article{Sittichockechaiwut2009UseOR,
  title={Use of rapidly mineralising osteoblasts and short periods of mechanical loading to accelerate matrix maturation in 3D scaffolds.},
  author={Anuphan Sittichockechaiwut and Andrew M Scutt and Anthony J Ryan and Lynda F. Bonewald and Gwendolen C Reilly},
  journal={Bone},
  year={2009},
  volume={44 5},
  pages={822-9}
}
MLO-A5 cells are a fully differentiated osteoblastic cell line with the ability to rapidly synthesise mineralised extracellular matrix (ECM). We used MLO-A5 cells to develop a system for studying the mechanical modulation of bone matrix formation in 3D using a cyclic compressive loading stimulus. Polyurethane (PU) open cell foam scaffolds were seeded with… CONTINUE READING