Use of random systematic mutagenesis to generate viable human rhinovirus 14 chimeras displaying human immunodeficiency virus type 1 V3 loop sequences.

@article{Smith1994UseOR,
  title={Use of random systematic mutagenesis to generate viable human rhinovirus 14 chimeras displaying human immunodeficiency virus type 1 V3 loop sequences.},
  author={Allen D. Smith and Damon A Resnick and Aixia Zhang and Sheila C. Geisler and Eddy Arnold and Gail Ferstandig Arnold},
  journal={Journal of virology},
  year={1994},
  volume={68 1},
  pages={575-9}
}
Random systematic mutagenesis was used to generate a library of human rhinovirus 14 chimeras that each display a segment from the V3 loop of human immunodeficiency virus type 1. The sequence XXIGPGRAXX, where X could be any of the 20 amino acids, was inserted at the neutralizing immunogenic site II of human rhinovirus 14 between VP2 residues 159 and 160. Twenty-five unique chimeric viruses were isolated, and the identity of their randomized residues was determined. A nonrandom amino acid… CONTINUE READING
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