Use of proteoliposomes to generate phage antibodies against native AMPA receptor.

@article{Jespersen2000UseOP,
  title={Use of proteoliposomes to generate phage antibodies against native AMPA receptor.},
  author={Lene Jespersen and Arja Kuusinen and Ariel Orellana and Kari Kein{\"a}nen and Jan Engberg},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 5},
  pages={1382-9}
}
To isolate antibodies against ionotropic glutamate receptors (GluRs), we prepared a phage antibody library from mice immunized with proteoliposomes containing purified alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), a selective GluRD receptor. Specific binders were selected by repeated rounds of affinity panning against immobilized GluRD liposomes. Using this approach, we obtained a panel of high-affinity antibody fragments that immunoprecipitated both recombinant and native… CONTINUE READING
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