Use of polymerase chain reaction to diagnose bovine leukemia virus infection in calves at birth.

@article{Agresti1993UseOP,
  title={Use of polymerase chain reaction to diagnose bovine leukemia virus infection in calves at birth.},
  author={Alessandra Agresti and Wilma Ponti and Mara Rocchi and Raffaella Meneveri and Anna Marozzi and D Cavalleri and Ezio Peri and Guido Poli and Enrico Ginelli},
  journal={American journal of veterinary research},
  year={1993},
  volume={54 3},
  pages={373-8}
}
A specific polymerase chain reaction (PCR) assay was devised, allowing detection of 1 bovine leukemia virus (BLV)-infected cell in 10(4) bovine lymphocytes. The efficacy of field application of the developed method was verified by evaluating the rate of viral transmission to calves from infected cows, whether they have persistent lymphocytosis. With this objective, 43 calves were simultaneously tested at birth and at 6 months of age for viral antibodies in serum and for proviral DNA in… CONTINUE READING

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