Use of polyethyleneglycol for porcine islet cryopreservation.

  title={Use of polyethyleneglycol for porcine islet cryopreservation.},
  author={Burgoa Monroy and Jiry Honiger and Sylviane Darquy and G{\'e}rard Reach},
  journal={Cell transplantation},
  volume={6 6},
The aim of this work was to determine whether polyethylene glycol 20000 Da (PEG) could be used as protective agent in porcine islet cryopreservation. Cryopreservation was performed on 1-wk cultured pig islets and consisted in an overnight storage in liquid nitrogen. In a first set of experiments, we compared the in vitro function of PEG-cryopreserved islets to that of porcine islets cryopreserved under the standard procedure using dimethylsulfoxide (DMSO), by incubating the islets over 45 min… CONTINUE READING