Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

@article{Stahl1988UseOP,
  title={Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.},
  author={David A. Stahl and Berdena Flesher and Heather Mansfield and L J Montgomery},
  journal={Applied and environmental microbiology},
  year={1988},
  volume={54 5},
  pages={1079-84}
}
To address the long-standing need for more precise descriptions of natural microbial ecosystems, 16S rRNAs were used to track certain species and phylogenetically coherent groups of microorganisms in their natural setting without culturing. Species- and group-specific 16S rRNA-targeted oligonucleotide hybridization probes were developed to enumerate various strains of Bacteroides succinogenes and Lachnospira multiparus-like organisms in the bovine rumen before, during, and after perturbation of… CONTINUE READING
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