Use of peptides from p21 (Waf1/Cip1) to investigate PCNA function in Xenopus egg extracts.

@article{Mattock2001UseOP,
  title={Use of peptides from p21 (Waf1/Cip1) to investigate PCNA function in Xenopus egg extracts.},
  author={H Mattock and Pedro Jares and Daniella I Zheleva and D. P. Lane and E Vicky Warbrick and J. Julian Blow},
  journal={Experimental cell research},
  year={2001},
  volume={265 2},
  pages={242-51}
}
Cell-free systems derived from unfertilized Xenopus eggs have been particularly informative in the study of the regulation and biochemistry of DNA replication. We have developed a Xenopus-based system to analyze proliferating cell nuclear antigen (PCNA)-specific effects on the functional properties of egg extracts. To do this, we have coupled peptides derived from p21 (Waf1/Cip1) to beads and used these to deplete PCNA from Xenopus egg extracts. The effect on various aspects of DNA replication… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 49 references

The puzzle of PCNA's many partners.

BioEssays : news and reviews in molecular, cellular and developmental biology • 2000

Quanti - tation and subcellular localization of proliferating cell nuclear antigen ( PCNA / cyclin ) in oocytes and eggs of Xenopus laevis

K. Shibahara, B. Stillman
Exp . Cell Res • 1999

Selective killing of transformed cells by cyclin/cyclin-dependent kinase 2 antagonists.

Proceedings of the National Academy of Sciences of the United States of America • 1999
View 1 Excerpt

Activity and nature of p21(WAF1) complexes during the cell cycle.

Proceedings of the National Academy of Sciences of the United States of America • 1998
View 1 Excerpt

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