Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166.

@article{Sommerfelt1992UseON,
  title={Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166.},
  author={Halvor Sommerfelt and Harleen M S Grewal and Wim Gaastra and Ann Mari Svennerholm and M K Bhan},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 7},
  pages={1823-8}
}
We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface antigen 4 (CS4), or putative colonization… CONTINUE READING