Use of nile red as a rapid measure of lipid content in ciliates.

  title={Use of nile red as a rapid measure of lipid content in ciliates.},
  author={T. A. Cole and A. K. K. Fok and Masaki Ueno and Richard D M Allen},
  journal={European journal of protistology},
  volume={25 4},
We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitative estimates of the relative changes in the lipid content of cells of varying culture ages were obtained using fluorescence microscopy, while semiquantitative determinations were obtained by measuring the total fluorescence from the emission… CONTINUE READING
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