Use of nile red as a rapid measure of lipid content in ciliates.

@article{Cole1990UseON,
  title={Use of nile red as a rapid measure of lipid content in ciliates.},
  author={T. A. Cole and A. K. K. Fok and Masaki Ueno and Richard D M Allen},
  journal={European journal of protistology},
  year={1990},
  volume={25 4},
  pages={361-8}
}
We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitative estimates of the relative changes in the lipid content of cells of varying culture ages were obtained using fluorescence microscopy, while semiquantitative determinations were obtained by measuring the total fluorescence from the emission… CONTINUE READING
Highly Cited
This paper has 20 citations. REVIEW CITATIONS
8 Citations
21 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 21 references

Mechanism of nile red transfer from o / w emulsions as carriers for passive drug targeting to peritoneal macrophages in vitro

  • C. HaynesL., J. ChoM.
  • Int . J . Parmaceutics
  • 1988

Useof nile red in the detection of cholesteryl ester accumulation in acidlipase deficient fibroblasts

  • W. Brown, WarfelJ., P. Greenspan
  • Arch . Path . Lab . Med .
  • 1988

Fluorometric determination of the neutral lipid content of microalgal cells using nile red

  • E. CookseyK., B. GuckertJ., A. WilliamsS., R. CallisP.
  • J . Microbiol . Methods
  • 1987

Lipid distributions in mouse eggs and preimplantation embryos as revealed by the vital fluorescent dye nile red

  • E. BattenB.
  • 1987

Nile red as a polaritysensitive fluorescenceprobe of hydrophobic protein surfaces

  • L. SackettE., J. Wolff
  • Anal . Biochem .
  • 1987

Nilered : a selective fluorescent stain for intracellular lipid droplets

  • A. K. Fok, R. D. Allen, GreenspanP., P. MayerE., D. FowlerS.
  • J . Cell BioI .
  • 1985

Similar Papers

Loading similar papers…