Use of nested PCR to identify charred human remains and minute amounts of blood.

@article{Strom1998UseON,
  title={Use of nested PCR to identify charred human remains and minute amounts of blood.},
  author={Charles M. Strom and Svetlana Rechitsky},
  journal={Journal of forensic sciences},
  year={1998},
  volume={43 3},
  pages={696-700}
}
Reliable single cell PCR requires nested or heminested PCR and careful optimization of conditions. This report describes the successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains and a minute quantity of blood that were unsuitable for standard PCR. Use of nested PCR allowed the blood and burned tissue to be identified as human female. Analysis of two PCR length polymorphisms… CONTINUE READING

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