Use of multiplex polymerase chain reaction-based assay to conduct epidemiological studies on bovine hemoparasites in Mexico.

@article{Figueroa1993UseOM,
  title={Use of multiplex polymerase chain reaction-based assay to conduct epidemiological studies on bovine hemoparasites in Mexico.},
  author={Julio V. Figueroa and J Antonio Alvarez and Juan A Ramos and Carlos A. Vega and Gerald M Buening},
  journal={Revue d'elevage et de medecine veterinaire des pays tropicaux},
  year={1993},
  volume={46 1-2},
  pages={71-5}
}
A study was conducted to test the applicability of a Polymerase Chain Reaction (PCR)-based approach for the simultaneous detection of the bovine hemoparasites Babesia bigemina, B. bovis and Anaplasma marginale. Bovine blood samples from cattle ranches of a previously determined enzootic zone in the Yucatan Peninsula of Mexico, were collected from peripheral blood and processed for PCR analysis. Blood samples were subjected to DNA amplification by placing an aliquot in a reaction tube containing… CONTINUE READING