Use of multiple isotope effects to study the mechanism of 6-phosphogluconate dehydrogenase.

@article{Rendina1984UseOM,
  title={Use of multiple isotope effects to study the mechanism of 6-phosphogluconate dehydrogenase.},
  author={Alan Ralph Rendina and Jeffrey D. Hermes and Wm. Cleland},
  journal={Biochemistry},
  year={1984},
  volume={23 25},
  pages={
          6257-62
        }
}
The multiple isotope effect method of Hermes et al. [Hermes, J. D., Roeske, C. A., O'Leary, M. H., & Cleland, W. W. (1982) Biochemistry 21, 5106-5114] has been used to study the mechanism of the oxidative decarboxylation catalyzed by 6-phosphogluconate dehydrogenase from yeast. 13C kinetic isotope effects of 1.0096 and 1.0081 with unlabeled or 3-deuterated 6-phosphogluconate, plus a 13C equilibrium isotope effect of 0.996 and a deuterium isotope effect on V/K of 1.54, show that the chemical… CONTINUE READING
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