Use of multiple displacement amplification to increase the detection and genotyping of trypanosoma species samples immobilized on FTA filters.

@article{Morrison2007UseOM,
  title={Use of multiple displacement amplification to increase the detection and genotyping of trypanosoma species samples immobilized on FTA filters.},
  author={Liam J. Morrison and G Mccormack and Lindsay Sweeney and A C L{\'e}kan{\'e} Likeufack and Philippe Truc and Charles M. R. Turner and Andy Tait and Annette MacLeod},
  journal={The American journal of tropical medicine and hygiene},
  year={2007},
  volume={76 6},
  pages={1132-7}
}
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias. Multiple displacement amplification (MDA) amplifies DNA with a simple in vitro step and was evaluated on mouse blood samples on FTA filter cards with known numbers of Trypanosoma brucei parasites. The data showed a 20-fold increase in the number of PCRs possible per sample, using primers diagnostic for… CONTINUE READING