Use of multiple PCR primer sets for optimal detection of human papillomavirus.

  title={Use of multiple PCR primer sets for optimal detection of human papillomavirus.},
  author={Frank Karlsen and Mina Kalantari and Andrew J. Jenkins and E O Pettersen and Gunnar Balle Kristensen and Ruth Holm and Bo M. Johansson and Bj\orn M Hagmar},
  journal={Journal of clinical microbiology},
  volume={34 9},
Using multiple PCR primer sets, we tried to optimize the detection of human papillomavirus (HPV) in DNA samples isolated from 361 frozen biopsy specimens from patients with invasive cervical carcinomas. The HPVs detected were placed into three distinct groups, including group I/Inex at Telelab (Skien, Norway) and group Ineg and group II at the Norwegian Radium Hospital (Oslo, Norway). The consensus primer sets were Oli-1b-oli-2i, My09-My11, Gp5-Gp6, and Gp(5+)-Gp6+ from the HPV L1 gene and CpI… CONTINUE READING

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